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KMID : 0624620080410040300
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BMB Reports
2008 Volume.41 No. 4 p.300 ~ p.304
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DAZL binds to the transcripts of several Tssk genes in germ cells
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Zeng Mei
Deng Wenqian
Wang Xinying
Qiu Weimin
Liu Yanyan
Sun Huaqin
Tao Dachang
Zhang Sizhong
Ma Yongxin
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Abstract
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The Dazl gene encodes a germ-cell-specific RNA-binding protein which is essential for spermatogenesis. It has been proposed that this protein (DAZL) binds to RNA in the cytoplasm of germ cells and controls spermatogenesis. Using the specific nucleic acids associated with proteins (SNAAP) technique, we identified 17 target mRNAs bound by mDAZL. Among these transcripts, we focused on TSSK2, which encodes a testis-specific serine/threonine kinase. To date, five TSSK family members have been cloned, and all are exclusively expressed in the testis. We demonstrated that in addition to the TSSK1 3’UTR , the 3’UTRs of TSSKs 2 and 4 were bound by human and mouse DAZL, and that human DAZL (hDAZL) bound to the 3’UTR of human TSSK5 (hTSSK5). Our results suggest that the Dazl gene may play different roles in human and mouse spermatogenesis by regulating different members of the downstream gene family. [BMB reports 2008; 41(4): 300-304]
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KEYWORD
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Dazl gene, RNA-binding protein, Spermatogenesis, Target mRNAs, TSSK
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